A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable minisatellites.

摘要: The issue of whether genetic exchange occurs at a significant frequency in natural populations Trypanosoma brucei is controversial and one the arguments against high has been apparent lack host infections with mixtures trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) within coding regions three genes have identified PCR based methods developed for detecting variation these loci using crude lysates infected blood as templates. Initial analysis, primers flanking repeats, DNA from two cloned stocks parasite shown that fragments different size were amplified each stock. Analysis inheritance into F1 progeny crosses demonstrated alleles segregated Mendelian manner. independently consistent their localisation on chromosomes. series isolates tsetse flies showed highly variable giving heterozygosities 94% identification 12 distinct sample 17 isolates. In order to determine are heterogeneous terms genotype, allelic was examined uncloned samples isolated Kiboko, Kenya Lugala, Uganda. A proportion (36% Lugala 47% Kiboko) contained more than or thus demonstrating genotype trypanosomes. This established, unequivocally, by generating lines determining clone; isolate (927) seven genotypes possible combinations locus. These results indicate possibility frequent field, they imply mammalian hosts must contain demonstrate advantages analysis population structure T. brucei.