Physicochemical characterization of anionic lipid-based ternary siRNA complexes.
作者： Mamta Kapoor , Diane J. Burgess
DOI: 10.1016/J.BBAMEM.2012.03.013
关键词: Ternary operation 、 Particle size 、 Calcium 、 Surface charge 、 Liposome 、 Transfection 、 In vitro 、 Chemistry 、 Biophysics 、 Chromatography 、 Biological activity
摘要: Physicochemical characterization is a useful tool in understanding lipoplex assemblies and their correlation to biological activity. Anionic lipid-based ternary siRNA complexes composed of anionic liposomes (DOPG/DOPE), calcium ions siRNA, have recently been shown be safe efficient breast cancer cell culture model. In the present work, effects various formulation parameters such as liposome composition (DOPG/DOPE ratio) lipid/Ca2+/siRNA molar charge ratio, on physicochemical attributes (particle size, surface charge, loading efficiency serum stability) these lipoplexes were evaluated. Particle stability correlated with vitro silencing lipoplexes. For example, large particles (5/2.5/1 showed less while absolute high (1.3/2.5/1 ratio), exhibited maximum cells. The properties also indicated that exists complexed and/or encapsulated form within lipoplexes, depending lipid/siRNA ratio. Based studies model representing lipid-siRNA association prepared under conditions proposed. can utilized estimate activity understand morphology.
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