Rapid detection of drugs in biofluids using atmospheric pressure chemi/chemical ionization mass spectrometry.

摘要: We have demonstrated that, with simple pH adjustment, volatile drugs such as methamphetamine, amphetamine, 3,4-methylenedioxymethamphetamine (MDMA), ketamine, and valproic acid could be analyzed rapidly from raw biofluid samples (e.g. urine serum) without dilution, or extraction, using atmospheric pressure ionization. The ion source was a variant type of chemical ionization (APCI) that used dielectric barrier discharge (DBD) to generate the metastable helium gas reagent ions. sample solution loaded in disposable glass pipette, compounds were purged by nitrogen reacted gas. electrodes DBD arranged way generated glow confined within tube not exposed analytes. A needle held at 100–500 V placed between ion-sampling orifice guide analyte ions into mass spectrometer. After adjustment sample, amphiphilic form water-insoluble oil, which concentrated on liquid surface. By gentle heating increase evaporation rate, rapid sensitive detection these serum achieved less than 2 min for each sample. Copyright © 2009 John Wiley & Sons, Ltd.