Oligosaccharides containing glucose and mannose in glycoproteins of the thyroid gland

作者: R. J. Staneloni , L. F. Leloir

DOI: 10.1073/PNAS.75.3.1162

关键词: Amino acidGlycoproteinOligosaccharideChromatographyMannoseBorohydrideChemistryPolyisoprenyl Phosphate OligosaccharidesPronaseBiochemistryMonosaccharide

摘要: Abstract The glucose-containing oligosaccharides formed by calf thyroid slices incubated with radioactive glucose were studied. A compound soluble in chloroform/methanol/water, 1:1:0.3 (vol/vol), was found that indistinguishable from the previously described dolichyl diphosphate oligosaccharide liver microsomes. Glycopeptides prepared treating glycoproteins pronase, amino acids removed alkaline borohydride, and products examined paper electrophoresis chromatography. saccharide equal to which occurs could not be detected but seemed smaller about three five monosaccharide residues. The same results obtained direct treatment of borohydride.

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