TanshinoneIIA Alleviates Inflammatory Response and Directs Macrophage Polarization in Lipopolysaccharide-Stimulated RAW264.7 Cells.
作者： Shan Gao , Yili Wang , Dan Li , Yuying Guo , Meifeng Zhu
DOI: 10.1007/S10753-018-0891-7
关键词: Inflammatory response 、 Cell morphology 、 Cytokine 、 Macrophage polarization 、 Cell biology 、 Lipopolysaccharide 、 M2 Macrophage 、 ARG1 、 Chemistry 、 Flow cytometry
摘要: TanshinoneIIA (TanIIA) has been demonstrated to possess numerous biological effects. However, the specific effect of TanIIA on macrophage polarization not reported. In this study, it was revealed that might play a pivotal role in polarization. As our results indicated, cell morphology changed RAW264.7 cells which were treated with LPS or LPS/TanIIA (0.1 μM, 1 μM, 10 μM). Subsequently, pro-inflammatory cytokine TNF-α and anti-inflammatory IL-10 measured by ELISA kits. Furthermore, enhanced expression M2 markers (Arg1 FIZZ1) decreased associated M1 (iNOS IL-1β). Increased CD206 also detected flow cytometry TanIIA-treated groups. Mechanistically, modulated ameliorating mitochondrial function regulating TLR4-HMGB1/CEBP-β pathway. addition, increased miR-155 observed incubated effectively inhibited TanIIA. Taken together, suggested inhibits inflammatory response promotes toward an phenotype, provides new evidence for anti-inflammation activity
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