Chronic ethanol promotes the neuronal differentiation of NG108-15 cells independently of toxin-sensitive G-proteins.

摘要: The ability of ethanol to promote neuronal differentiation NG108-15 mouse neuroblastoma x rat glioma hybrid cells was investigated using morphological, biochemical and electrophysiological techniques. Ethanol concentration-(10-200 mM) time-(1 h-3 days) dependently reduced cell proliferation, but increased acetylcholinesterase (AChE) activity protein content. Chronic (200 also time-dependently voltage-sensitive Ca(2+) currents in the cells. Similar effects were obtained with chronic treatment standard differentiating agents sodium butyrate or forskolin. primary alcohols (0.1-200 varying chain length all proliferation content AChE potency order butanol>propanol>ethanol>methanol. cholera toxin (50 ng ml(-1)) pertussis did not induce cells, nor it modify 50 200 mM on cellular however abolish agonist-stimulated adenylyl cyclase whereas abolished receptor-mediated inhibition activity. Furthermore, inhibitors kinase C (GF 109203X, 5 μM), A (H-89, 10 μM) Ca(2+)/calmodulin-dependent II (KN-62, 3 failed These experiments indicate that is able independently toxin-sensitive G-proteins some kinases.