Determination of the Relationship Between T Cell Responsiveness and the Number of MHC-Peptide Complexes Using Specific Monoclonal Antibodies
作者： Philip A. Reay , Kiyoshi Matsui , Katherine Haase , Christoph Wulfing , Yueh-Hsiu Chien
DOI: 10.4049/JIMMUNOL.164.11.5626
关键词: Biochemistry 、 Lymphokine 、 Epitope 、 MHC restriction 、 Major histocompatibility complex 、 T cell 、 Chemistry 、 Peptide 、 Monoclonal antibody 、 Peptide sequence 、 Molecular biology
摘要: We describe the generation of three mAbs that recognize complex class II MHC molecule IEk bound to a peptide derived from carboxyl terminus moth cytochrome c (residues 95-103). Reactivities these are sensitive single alterations in sequence both helices and peptide. The epitopes reagents distinct but overlap substantially. One specifically blocks lymphokine release by T cells responsive this not others. have used another examine how number complexes on an APC is related its ability stimulate cells. find 200-400 per cell necessary sufficient induce degree stimulation, whereas maximum stimulation achieved only if more than 5000 present. analysis indicates activation stochastic process.
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