Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease.

摘要: Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils monocytes. The activated release myeloperoxidase (MPO), specific enzyme with strong oxidative activity. aim this study was to evaluate MPO concentrations plasma peritoneal fluid (PF) horses colic check hypothesis that these would be higher case obstruction than cases nonstrangulating disease. By using enzyme-linked immunosorbent assay for equine MPO, we determined admitted clinic colic. Horses or large intestine (NSLI SLI), small (SSI), inflammatory bowel disease (IBD) were compared healthy horses. SLI, SSI, IBD had significantly levels PF did those other 2 groups. mean level NSLI High values indicated necrotic bowel. These results show neutrophil activation occurs during marker severity