Immunochemical Quantitation of Cell Bound C4

摘要: A method is described for the determination of absolute amounts human (Hu) and guinea pig (GP) C4 bound to cell surfaces. The test based on inhibition anti-C4 antibody by C4. be measured added a standard amount residual activity determined its capacity sensitize appropriate indicator cells (EC4 h or EC4 gp ) lysis GPC. By comparing observed with inhibition-of-lysis curves obtained known antigen, antigen can estimated. simple, rapid reproducible detect nanogram Guinea hepatoma harvested from peritoneal cavity pigs contained approximately 10 5 GPC per whereas tumor sensitized anti-Forssman specific antitumor 2 3 × 6 cell. content Hu serum pool GP sera was quantitative precipitin analysis found 942 ± 260 µg 1106 106 µg/ml, respectively. Based this average 12 718 61 µg/ml (range 577 µg/ml) in paper.