Quantitation of antibody binding to cell surface antigens by X-ray fluorescence spectrometry

作者: Eric M. Goldin , Peter M. Corry , Christine H. Granatek

DOI: 10.1016/0005-2736(79)90251-7

关键词:

摘要: Abstract An X-ray spectrometric method has been developed to quantitate antibody binding whole cell surfaces in order obtain a distribution of within population cells. The involves incubation target cells with ferritin-labeled antibody. Analysis prepared samples modified transmission electron microscope an detector and data analysis equipment, yields quantitative results on the labeled individual anti-2,4-dinitrophenol serum Chinese hamster ovary attached 2,4-dinitrophenol haptens was measured by spectrometry. Measurements hapten radioisotopic marker correlated determination bound use synchronized metaphase G1 phases cycle permitted investigations into per unit surface area. among given related area

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