Quantitative studies on the binding of antibody to the surface of HeLa cells.

摘要: The binding of horse antibody to viable HeLa cells was studied using mixed immune and normal sera labeled with different iodine radioisotopes, a technique which permits precise correction for nonspecific adsorption. association constant the reaction, maximum antibody-binding capacity cells, content serum were determined two types titration: concentration titrated serum, cells. titration data conformed closely linear equations derived from mass action law. When applied antigenantibody reactions are heterogeneous respect affinity, these have advantageous properties: 1) plots remain within known degree error can be made small, 2) K value obtained slope ordinate intercept represents weighted mean values each affinity subspecies in population.