Application of the Paired Label Radioantibody Technique to Tissue Sections and Cell Smears

摘要: Summary A micro-scale modification of the paired label radioantibody technique has been developed to determine reactivity antiserum globulin with tissue sections or cell smears. mixture 125 I-labeled IgG from and 131 normal serum is applied on test specimens fixed microscope coverslips. After incubation for 60 min at room temperature, are washed assayed radioactivity due I I. The degree was expressed by uptake quotient, quotient specific over nonspecific uptake. method sensitive reproducible under conditions given. It suitable comparing a large number particularly useful where materials, tissues cells, available only in limited quantities. Various factors involved reaction—reaction time, concentration antibody globulin, area thickness sections, antigen content absorption sediments—were evaluated detail.