Fractalkine, a novel chemokine in rheumatoid arthritis and in rat adjuvant-induced arthritis.
作者： Jeffrey H. Ruth , Michael V. Volin , G. Kenneth Haines , Drew C. Woodruff , Kenneth J. Katschke
DOI: 10.1002/1529-0131(200107)44:7<1568::AID-ART280>3.0.CO;2-1
关键词: Dendritic cell 、 Chemokine 、 Arthritis 、 Macrophage 、 Medicine 、 Synovial fluid 、 CX3CR1 、 Monocyte 、 Monocyte chemotaxis 、 Immunology
摘要: Objective To examine the expression of novel CX3C chemokine fractalkine (Fkn) and its receptor (CX3CR1) in rheumatoid arthritis (RA) rat adjuvant-induced (AIA), a model RA. Methods Immunohistochemistry, flow cytometry, enzyme-linked immunosorbent assay (ELISA), reverse transcriptase–polymerase chain reaction (RT-PCR), chemotaxis assays were used. Results In AIA, synovial tissue (ST) macrophages, fibroblasts, endothelial cells, dendritic cells Fkn immunopositive, whereas lymphocytes did not significantly express Fkn. Significant staining for CX3CR1 was found ST only small percentage stained AIA. We immunolocalized to RA cells. also intense macrophage cell ST. Flow cytometry analysis fluid (SF) peripheral blood revealed greater monocytes expressing compared with T By ELISA, we elevated soluble (sFkn) levels SF from patients osteoarthritis or other forms arthritis. RT-PCR, enhanced mRNA on day 18 time pronounced inflammation joint. Soluble Fkn–depleted showed decreased chemotactic activity sham-depleted SF. Conclusion These results indicate that are both expressed sFkn is up-regulated SF. Furthermore, our data suggest new role monocyte inflamed
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