12β-Hydroxylation of digitoxin by Digitalis lanata cells. Production of deacetyllanatoside C in a 20-litre airlift bioreactor
作者： Wolfgang Kreis , Weihua Zhu , Ernst Reinhard
DOI: 10.1007/BF01024512
关键词: Bioreactor 、 Digitalis lanata 、 Digitalis grandiflora 、 Hydroxylation 、 Chromatography 、 Chemistry 、 Airlift 、 Digitoxin 、 Biotransformation 、 Fermentation
摘要: Digitalislanata cell lines obtained via aggregate cloning have been characterized with regard to their growth and ability form deacetyllanatoside C from digitoxin. Cell line W.1.4 achieved 12β-hydroxylation rates as high 200 mg/L d. It was thus used in biotransformation experiments on a 20-litre scale. Six fermentor runs were performed, the best of which yielded 13.2 g C.
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