Target-programmed and autonomous proximity binding aptasensor for amplified electronic detection of thrombin.

摘要: Abstract The development of sensitive and simple approaches capable monitoring trace amounts protein biomarkers is appealing for disease diagnosis treatment. Towards this end, we have developed an electrochemical sensing platform detection by using thrombin as the model target molecules via a target-programmed proximity binding amplification approach. to aptamer sequences in partial dsDNA duplex probes induces release ssDNA trigger strands, which catalyze subsequent assembly formation many methylene blue (MB)-tagged proximate DNA motifs with presence fuel strands through cascaded toehold-mediated strand displacement reactions. Due proximity-binding effect, these MB-tagged anneal capture on sensor surface significantly enhanced stability against corresponding single component counterpart, thereby pulling MB tags close generating substantially amplified signal responses determination down 23.6 pM. In addition, such aptasensor can specifically discriminate from other interference proteins, also be utilized monitor diluted serum samples, demonstrating its great potential proteins early diagnosis.