Pre-operative intracellular glutathione levels of peripheral monocytes as a biomarker to predict survival of colorectal cancer patients

摘要: The ability to predict anti-tumor immune responses at local tumor growing sites using only peripheral blood specimens would be helpful in determining therapeutic options for patients with solid tumors. Here, we show that the glutathione intracellular content (icGSH) of monocytes (Mo) correlates positively T cell infiltration within islets and overall survival colorectal carcinoma. IcGSH redox status was determined CD14+ Mo prior surgery by staining monochlorobimane. tumor-infiltrating cells (TIL) were quantified as CD45RO+ resected tumors paraffin sections. A positive association found between GSH index TIL (P < 0.001). 50% cut-off value index, is determinant presence or absence islets, calculated almost 0.7 through logistic regression analysis. a ≥0.7 termed reductive (R)-Mo, those <0.7 designated oxidative (O)-Mo. Cox’s proportional hazards analysis R-Mo O-Mo surgery, TIL, correlate significantly rate stage II III patients. Kaplan–Meier also showed significant correlation. These results indicate icGSH useful biomarker parameter better understanding host/tumor relationship thereby enabling development an individual patient-oriented strategy.