Effect of varying pH on protein composition and yield of amaranth seed (Amaranthus blitum).

摘要: The isolation procedure of the seed proteins Amaranthus blitum have been analyzed at different pH conditions. Qualitative studies were carried out by using electrophoretic technique sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Mainly four protein fractions i.e. albumin, globulin, prolamin and glutelin obtained. Protein isolates prepared (a) extraction alkaline (9, 10, 11 12) precipitation atpH 5 (b) 9 atdifferentpH (4, 5, 6 7). composition isolates, method (a), depended on pH. isolate extracted 8 was mainly composed albumin whereas 9, 10 showed presence glutelin. Electrophoretic pattern had five major bands with molecular weight 30, 45, 72, 84 90 kDt respectively. increase led to in yield. With obtained variable composition. At 7 prolamine present, 4, all 4 precipitated. According quantitative estimation flour contents 26.4, 25, 5.81 42.7%, results suggest that could be controlled