Effects of Nandrolone Stimulation on Testosterone Biosynthesis in Leydig Cells.
作者： Cristoforo Pomara , Rosario Barone , Antonella Marino Gammazza , Claudia Sangiorgi , Fulvio Barone
DOI: 10.1002/JCP.25272
关键词: In vivo 、 Nandrolone 、 Endocrinology 、 Steroidogenic acute regulatory protein 、 Downregulation and upregulation 、 Luteinizing hormone 、 Testosterone 、 Biology 、 CYP17A1 、 Hormone 、 Internal medicine
摘要: Anabolic androgenic steroids (AAS) are among the drugs most used by athletes for improving physical performance, as well aesthetic purposes. A number of papers have showed side effects AAS in different organs and tissues. For example, known to suppress gonadotropin-releasing hormone, luteinizing follicle-stimulating hormone. This study investigates nandrolone on testosterone biosynthesis Leydig cells using various methods, including mass spectrometry, western blotting, confocal microscopy quantitative real-time PCR. The results obtained show that levels increase at a 3.9 μM concentration return basal level 15.6 μM dose nandrolone. Nandrolone-induced increment was associated with upregulation steroidogenic acute regulatory protein (StAR) downregulation 17a-hydroxylase/17, 20 lyase (CYP17A1). Instead, 15.6 µM induced down-regulation CYP17A1. Further vivo studies based these data needed better understand relationship between disturbed homeostasis reproductive system impairment male subjects.
sci-hub.se 参考文章(36)
Farzad Gheshlaghi, Gholam Reza Masoumi, Mohaddaseh Behjati, Mohammad-Reza Piri-Ardakani, Parva Paydar, Cardiovascular manifestations of anabolic steroids in association with demographic variables in body building athletes. Journal of Research in Medical Sciences. ,vol. 20, pp. 165- 168 ,(2015)
Valentina Di Felice, Claudia Serradifalco, Luigi Rizzuto, Angela De Luca, Francesca Rappa, Rosario Barone, Patrizia Di Marco, Giovanni Cassata, Roberto Puleio, Lucia Verin, Antonella Motta, Claudio Migliaresi, Annalisa Guercio, Giovanni Zummo, Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells. Journal of Tissue Engineering and Regenerative Medicine. ,vol. 9, ,(2015) , 10.1002/TERM.1739
Saeed Shokri, Robert John Aitken, Mirabbas Abdolvahhabi, Farid Abolhasani, Fahimeh Mohammad Ghasemi, Iraj Kashani, Shahram Ejtemaeimehr, Shahin Ahmadian, Bagher Minaei, Mohammad Ali Naraghi, Mohammad Barbarestani, Exercise and Supraphysiological Dose of Nandrolone Deconoate Increase Apoptosis in Spermatogenic Cells Basic & Clinical Pharmacology & Toxicology. ,vol. 106, pp. 324- 330 ,(2009) , 10.1111/J.1742-7843.2009.00495.X
ALI NOORAFSHAN, SAIED KARBALAY-DOUST, FAKHRODIN MESBAH ARDEKANI, High doses of nandrolone decanoate reduce volume of testis and length of seminiferous tubules in rats. Apmis. ,vol. 113, pp. 122- 125 ,(2005) , 10.1111/J.1600-0463.2005.APM1130205.X
Marija M. Janjic, Natasa J. Stojkov, Silvana A. Andric, Tatjana S. Kostic, Anabolic-androgenic steroids induce apoptosis and NOS2 (nitric-oxide synthase 2) in adult rat Leydig cells following in vivo exposure. Reproductive Toxicology. ,vol. 34, pp. 686- 693 ,(2012) , 10.1016/J.REPROTOX.2012.10.003
Filippo Macaluso, Rosario Barone, Patrizia Catanese, Francesco Carini, Luigi Rizzuto, Felicia Farina, Valentina Di Felice, Do Fat Supplements Increase Physical Performance Nutrients. ,vol. 5, pp. 509- 524 ,(2013) , 10.3390/NU5020509
Ruth I. Wood, Reinforcing aspects of androgens. Physiology & Behavior. ,vol. 83, pp. 279- 289 ,(2004) , 10.1016/J.PHYSBEH.2004.08.012
D. S. Keeney, R. L. Sprando, B. Robaire, B. R. Zirkin, L. L. Ewing, Reversal of long-term LH deprivation on testosterone secretion and Leydig cell volume, number and proliferation in adult rats. Journal of Endocrinology. ,vol. 127, pp. 47- 58 ,(1990) , 10.1677/JOE.0.1270047
Magdalena Gorska, Antonella Marino Gammazza, Michal Aleksander Zmijewski, Claudia Campanella, Francesco Cappello, Tomasz Wasiewicz, Alicja Kuban-Jankowska, Agnieszka Daca, Alicja Sielicka, Urszula Popowska, Narcyz Knap, Jakub Antoniewicz, Takashi Wakabayashi, Michal Wozniak, None, Geldanamycin-Induced Osteosarcoma Cell Death Is Associated with Hyperacetylation and Loss of Mitochondrial Pool of Heat Shock Protein 60 (Hsp60) PLoS ONE. ,vol. 8, pp. e71135- ,(2013) , 10.1371/JOURNAL.PONE.0071135
S. Hyyppä, U. Karvonen, L. A. Räsänen, S. G. B. Persson, A. R. Pösö, Androgen Receptors and Skeletal Muscle Composition in Trotters Treated with Nandrolone Laureate Journal of Veterinary Medicine Series A-physiology Pathology Clinical Medicine. ,vol. 44, pp. 481- 491 ,(1997) , 10.1111/J.1439-0442.1997.TB01134.X