Charged Gram-positive species sequester and decrease the potency of pediocin PA-1 in mixed microbial settings

摘要: Antimicrobial peptides (AMPs) have gained attention recently due to increasing antibiotic resistance amongst pathogens. Most AMPs are cationic in nature and their preliminary interactions with the negatively charged cell surface is mediated by electrostatic attraction. This followed pore formation, which either receptor-dependent or -independent leads death. Typically, characterized killing activity using bioactivity assays determine host range degree of killing. However, binding independent from studies performed to-date attempted quantify peptide artificial membranes. Here, we use narrow-spectrum class IIa bacteriocin AMP pediocin PA-1 conjugated a fluorescent dye as probe monitor binding. We developed flow cytometry-based assay strength target non-target species. Through our assays, found strong positive correlation between charge Interestingly, also inverse zeta potential binding, coefficient for improved when only Gram-positives were considered. show effect presence protein, salt, polycationic species, other species on organism. conclude that highly well solutes, can decrease apparent potency, PA-1. Thus, these outcomes significant related mixed microbial settings such those gut microbiota.