Molecular Characterization of Reticuloendotheliosis Virus Insertions in the Genome of Field and Vaccine Strains of Fowl Poxvirus

摘要: Abstract Evidence of the widespread occurrence reticuloendotheliosis virus (REV) sequence insertions in fowl poxvirus (FPV) genome field isolates and vaccine strains has increased recent years. However, only those carrying a near intact REV provirus are more likely to cause problems field. Detection or protein expression from FPV stocks proven be technically difficult. The objective present study was evaluate current newly developed polymerase chain reaction (PCR) assays detect presence samples. second characterize among “variant” strains. With REV, FPV, heterologous REV-FPV primers, five four commercial vaccines were analyzed by PCR nucleotide analysis. Intact truncated 5′ long terminal repeat (LTR) sequences detected all strains, ind...