Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing

摘要: Helicobacter pylori (H. pylori) infection is highly prevalent in the human population and may lead to severe gastrointestinal pathology including gastric duodenal ulcers, mucosa associated tissue lymphoma adenocarcinoma. In recent years, an alarming increase antimicrobial resistance subsequently failing empiric H. eradication therapies have been noted worldwide, also many European countries. Therefore, rapid accurate determination of pylori's antibiotic susceptibility prior administration regimens becomes ever more important. Traditionally, detection H.pylori its done by culture phenotypic drug testing that are cumbersome with a long turn-around-time. Recent advances diagnostics provide new tools, like real-time polymerase chain reaction (PCR) line probe assays, diagnose certain antibiotics, directly from clinical specimens. Moreover, high-throughput whole genome sequencing technologies allow analysis pathogen's genome, thereby allowing identification mutations resistance. first part this review, we will give overview on currently available diagnostic methods for their implementation management. The second review focusses use next generation technology research. To end, conducted literature search original research articles English using terms "Helicobacter", "transcriptomic", "transcriptome", "next sequencing" "whole sequencing". This aimed bridge gap between current practice (histology, urease test, culture, PCR assays) potential laboratory settings order complement recommended management guidelines improve public health.