Construction of recombinant human type 5 adenoviruses expressing rodent IL-6 genes. An approach to investigate in vivo cytokine function.

摘要: The majority of biologic functions assigned to cytokines have been characterized by in vitro assay systems which may not necessarily reflect cytokine roles vivo. Recently, recombinant virus approaches allowed tissue-specific expression foreign gene products experimental animal models. We constructed human type 5 adenoviruses, deficient the E3 region genome, with incorporated rodent IL-6 cDNA that express significant levels biologically active on infection both and After i.p. injection, liver, spleen, peritoneum appear be primary sites expression, whereas lung bronchus are main after intratracheal instillation. Injection BALB/c mice murine rIL-6 causes an increase serum bioactive for up 6 days post-infection, similar changes seen animals infected control viruses. Coincident enhanced plasma IL-6, we detect raised hepatic-derived acute phase proteins. Associated liver at 7 note a fourfold splenomegaly expansion B T cell compartments, as well presence lymphoid aggregates liver. These morphologic had resolved 16 days. Our findings demonstrate adenoviruses expressing various could used transient pseudo-transgenic model investigate function