Interplay of DNA repair, homologous recombination, and DNA polymerases in resistance to the DNA damaging agent 4-nitroquinoline-1-oxide in Escherichia coli

作者: Ashley B. Williams , Kyle M. Hetrick , Patricia L. Foster

DOI: 10.1016/J.DNAREP.2010.07.008

关键词: BiologyDNA polymerase IVDNA replicationDNA polymerase IIProcessivityDNA polymerase deltaDNA polymerase muDNA polymeraseMolecular biologyDNA polymerase V

摘要: Escherichia coli has three DNA damage-inducible polymerases: polymerase II (Pol II), IV IV), and V V). While the in vivo function of Pol is well understood, precise roles replication repair are not as clear. Study these polymerases largely focused on their participation recovery failed forks, translesion synthesis, origin-independent replication. However, other recombination pathways E. have been extensively examined. This study investigated how coli's inducible various together to convey resistance 4-nitroquinoline-1-oxide (NQO), a damaging agent that produces blocking base adducts. The data suggest full this compound depends upon an intricate interplay among activities recombination. also new relationships between different process intermediates.

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