Factors affecting the biosynthesis and secretion of gentamicin

摘要: Abstract The biosynthesis of gentamicin (GM) in shake flask cultivation and a resting cell system were studied. Suitable amounts NaNO 3 , MgSO 4 NaCl, penicillin, fosfomycin, cephradine, α-ketoglutarate, biotin Tween 20 favoured the GM. ingredients optimum synthetic medium for are given. amount GM adsorbed on 1 g wet mycelia wall was 4775 20 000 U, respectively. Usually only 20–30% is excreted into broth during fermentation, whereas about 75% bound can be released by ultrasonic treatment. production increased 70% when 0.8 ml (GM depleted) added 25 fermentation at 90 h after inoculation.