SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.
作者： Y Takebe , M Seiki , J Fujisawa , P Hoy , K Yokota
DOI: 10.1128/MCB.8.1.466
关键词: Regulation of gene expression 、 Molecular biology 、 Biology 、 Chloramphenicol acetyltransferase 、 Cell culture 、 RNA 、 Gene expression 、 Expression cloning 、 Long terminal repeat 、 Virus
摘要: We developed a novel promoter system, designated SR alpha, which is composed of the simian virus 40 (SV40) early and R segment part U5 sequence (R-U5') long terminal repeat human T-cell leukemia type 1. The R-U5' stimulated chloramphenicol acetyltransferase (CAT) gene expression only when placed immediately downstream SV40 in sense orientation. alpha system was 1 or 2 orders magnitude more active than wide variety cell types, including fibroblasts lymphoid cells, capable promoting high level various lymphokine cDNAs. These features were incorporated into pcD-cDNA cloning vector originally by Okayama Berg.
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