Gene Delivery to Chondrocytes Using Adenovirus Vector
作者： Toshikazu Kubo , Yuji Arai , Kappei Kobayashi , Jiro Imanishi , Masaharu Takigawa
DOI: 10.1007/978-4-431-68497-8_8
关键词:
摘要: The objective of this study was to investigate the effects adenovirus vector (Ax-)mediated gene transduction E. coli β-galactosidase (LacZ) and transforming growth factor-β1 (TGF-β1) into a human chondrocyte-like cell line (HCS-2/8). expression transduced genes their periods were examined by 5-bromo-4-chloroindolyl-β-D-galactoside (X-gal) staining, Northern blotting, ELISA, Western blotting. To assess influence TGF-β1 transduction, mRNAs type II collagen, proteoglycan core protein, matrix metalloproteinase-3 (MMP-3), tissue inhibitor metalloproteinase-1 (TIMP-1) Staining with X-gal indicated that 99% cells. Expression in cells continued for at least 21 days. Transduction enhanced mRNA expressions collagen but suppressed MMP-3 These results indicate Ax is useful chondrocyte therapy, it could be an efficient mediator transduction.
springer.com
sci-hub.st 参考文章(39)
Frank L. Graham, Ludvik Prevec, Manipulation of adenovirus vectors. Methods of Molecular Biology. ,vol. 7, pp. 109- 128 ,(1991) , 10.1385/0-89603-178-0:109
P M van der Kraan, O J Arntz, W B van den Berg, H M van Beuningen, Transforming growth factor-beta 1 stimulates articular chondrocyte proteoglycan synthesis and induces osteophyte formation in the murine knee joint. Laboratory Investigation. ,vol. 71, pp. 279- 290 ,(1994)
S E Whitham, G Murphy, P Angel, H J Rahmsdorf, B J Smith, A Lyons, T J R Harris, J J Reynolds, P Herrlich, A J P Docherty, Comparison of human stromelysin and collagenase by cloning and sequence analysis Biochemical Journal. ,vol. 240, pp. 913- 916 ,(1986) , 10.1042/BJ2400913
Edward J Murray (Ed), Gene Transfer and Expression Protocols ,(1991)
Monique Adolphe, Biological Regulation of the Chondrocytes ,(1992)
Y Yamada, T Kimura, K J Doege, M Sasaki, Complete coding sequence and deduced primary structure of the human cartilage large aggregating proteoglycan, aggrecan. Human-specific repeats, and additional alternatively spliced forms. Journal of Biological Chemistry. ,vol. 266, pp. 894- 902 ,(1991) , 10.1016/S0021-9258(17)35257-2
T I Morales, A B Roberts, Transforming growth factor beta regulates the metabolism of proteoglycans in bovine cartilage organ cultures. Journal of Biological Chemistry. ,vol. 263, pp. 12828- 12831 ,(1988) , 10.1016/S0021-9258(18)37634-8
Masaharu Takigawa, Koji Tajima, Akihiro Kinoshita, Yoshiro Takano, Motomi Enomoto, Fujio Suzuki, Yoichi Mori, Hai Ou Pan, Establishment of a Clonal Human Chondrosarcoma Cell Line with Cartilage Phenotypes Cancer Research. ,vol. 49, pp. 3996- 4002 ,(1989)
Y Takebe, M Seiki, J Fujisawa, P Hoy, K Yokota, K Arai, M Yoshida, N Arai, SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Molecular and Cellular Biology. ,vol. 8, pp. 466- 472 ,(1988) , 10.1128/MCB.8.1.466
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)