Enhanced proliferation of murine T cell lines following interaction of Poly(Glu60, Phe40) (GPhe) and antigen-presenting accessory cells

摘要: Abstract The random copolymers (Glu 80 , Phe 20 ) n (GPhe ), 60 ,Phe 40 (GPhe), and 50 were compared for the capacity to augment proliferation of antigen-reactive murine T cell lines. GPhe GPhe, showed “augmenting” activity in order increasing potency. Phenylalanyl residues constituted a significant portion “active” determinant(s) polymers tested. High titer anti-GPhe (ascites fluid) inhibited augmentation by exogenous (IL-1 + rat-conditioned media (RCM)) driven proliferation, indicating that (a) antibodies binding specific active may have prevented critical GPhe-APC membrane interaction, and/or (b) “GPhe-anti-GPhe” complexes interfered with necessary “processing” APCs. Time course studies demonstrated appearance increased after addition occurred nominal antigen or RCM) had reached peak [ 3 H]thymidine incorporation ([ HT]). This suggested more than interaction was activity. Leupeptin, lysosomal protease inhibitor, which led conclusion must be “processed” APCs exhibit