Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity.

摘要: Monoclonal antibodies (McAb) were developed to the Mr 17,500 form of human recombinant interleukin 1, IL 1 beta. Four McAb have been identified that inhibit biological activity H34 and H67, at microgram/ml (6 X 10(-9) M), completely capacity ng/ml 10(-11) M) beta stimulate proliferation murine thymocytes or fibroblasts in vitro. H6 H21 are approximately 10-fold less potent, 10 micrograms/ml 10(-8) both assays. The do not a significant effect on alpha either assay. These block binding [125I]IL receptors mouse 3T3 affinity constants for range 10(9) 10(10) liters/mol. Competition studies suggest two nonoverlapping epitopes molecule recognized by McAb. recognize one epitope, H67 another. used together two-site ELISA detect sensitivity ELISA, which is 15 pg/ml (0.86 pM), approaches limit thymocyte assay quantitate E. coli LPS-stimulated monocyte culture supernatants (HMCS). level detected from eight donors ranged 1.7 5.6 ng/ml, with mean value 3 ng/ml. By comparison, gave levels HMCS fold higher when quantitated using as standard. This discrepancy bioassay was reflected three maximum stimulation compared beta, only 45% inhibition Thus, factors other than account 1-like supernatant measured bioassay. ILB1 allow first time-sensitive, accurate, convenient quantitation fluids specimens.