Antimicrobial peptides from Burkholderia arboris RAI16 inhibit diabetic wound pathogens

摘要: Abstract The occurrence of diabetic mellitus has affected millions populations worldwide with global estimation 6.4%, hence affecting 285 million adults in 2010. Foot ulceration and wound infection are major forms complications prevalence to which is due microbial infections. Approximately 20% moderate or severe foot ulcer lead some level amputation. In this study, we aimed isolate potential AMP produced by Burkholderia arboris RAI16, a bacterium previously isolated from the mucus L. terrestris. crude peptides B. exhibited significant antimicrobial activity against all 9 test microorganisms. However, size inhibition zones varied among microorganisms, indicating different susceptibility microorganisms peptides. Among species, largest zone was observed on P. mirabilis. Enzymatic digestion revealed that significantly treatment trypsin, protease Proteinase K, confirming proteinaceous nature compound. A total 120 fractions were collected ion exchange chromatography. only three (Fraction 41, 113 118) contain anionic active inhibitory conclusion, AMPs RAI16 showed effect Further investigations should be conducted identify provide an alternative solution for