Modifying the mouse: design and desire.
作者： Allan Bradley , Paul Hasty , Ann Davis , Ramiro Ramirez-Solis
DOI: 10.1038/NBT0592-534
关键词: Gene 、 Embryonic stem cell 、 Stem cell 、 Cell culture 、 Genetics 、 Gene targeting 、 Genetic marker 、 Biology 、 Genome 、 Germline
摘要: Genetic modification of endogenous genes in mice has become possible by applying gene targeting techniques to embryonic stem (ES) cells and using specific clones generate mice. Despite the experimental opportunities offered creation organisms with genetic changes, there are considerable technical obstacles which can confound routine implementation this technology. This review addresses some recent advances ability construct a variety modifications. These include an increased understanding basic cell biology vitro growth characteristics ES cells, facilitated germ line transmission manipulated on basis. The that used isolate "targeted" have been summarized, current status strategies successfully make very modifications genome discussed.
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