Development of a framework for genotyping bovine-derived Cryptosporidium parvum , using a multilocus fragment typing tool

作者: Emily J. Hotchkiss , Janice A. Gilray , Marnie L. Brennan , Robert M. Christley , Liam J. Morrison

DOI: 10.1186/S13071-015-1107-8

关键词: TypingGenotypingBiologyFragment (logic)GenotypeGeneticsCryptosporidium parvumMultilocus sequence typing

摘要: Background There is a need for an integrated genotyping approach C. parvum; no sufficiently discriminatory scheme to date has been fully validated or widely adopted by veterinary public health researchers. Multilocus fragment typing (MLFT) can provide good differentiation and relatively quick cheap perform. A MLFT tool was assessed in terms of its typeability, specificity, precision (repeatability reproducibility), accuracy ability genotypically discriminate bovine-derived Cryptosporidium parvum.

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