[6] Kinetics of slow and tight-binding inhibitors

摘要: Publisher Summary The interest in both slow and tight-binding inhibitors has been increasing, mainly, owing to their importance as chemotherapeutic agents, herbicides, transition state analogs (reaction intermediate analogs). Although the quantitative description of effects these poses special problems, it is worth emphasizing that there nothing intrinsically different between classical inhibitors. Usually, derivation kinetic equations for nonclassical carried out following way: first, an inhibition mechanism proposed; second, some simplifying assumptions are set up; and, third, corresponding deduced. On other hand, when constants a new (slow and/or tight-binding) inhibitor be determined, procedure used: one or two alternative mechanisms considered; fitted experimental data; finally, model giving best fit accepted correct taken real values. There extensive reviews about this type inhibition, which include aspects: review by Williams Morrison Walsh binding Thus, present chapter discusses aspects have received less attention well developments, concerning kinetics more general mechanism, validity commonly used assumptions, determine constants, recent models analysis inhibition.