Presence of ChAT mRNA and a very marked α7nAChR immunoreaction in the synovial lining layer of the knee joint

摘要: Abstract Aims The aim was to examine if there is evidence of acetylcholine (ACh) production within the synovial lining layer and pattern α7nAChR expression in layer. This relevance clinically as it becomes thickened response both rheumatoid arthritis (RA) osteoarthritis (OA) has been shown produce proteases that are involved cartilage destruction. Main methods Synovial tissue specimens from knee joint patients with RA OA undergoing prosthetic surgery were examined. In situ hybridization immunohistochemistry used for evaluation ChAT reaction patterns. Immunohistochemistry utilized demonstration activity α7nAChR. Key findings There mRNA reactions patient categories. On other hand, no immunoreactions detected a very marked immunoreaction. Significance potential ACh reactions. However, level apparently low. It thus possible down-regulation but an apparent upregulation Based on knowledge non-neuronal cholinergic system can have anti-inflammatory effects, low be drawback arthritic joints.