The anti-apoptotic BAG3 protein is expressed in lung carcinomas and regulates small cell lung carcinoma (SCLC) tumor growth.

摘要: // Gennaro Chiappetta 1,* , Anna Basile 2,3,* Antonio Barbieri 4 Antonia Falco 2,3 Alessandra Rosati Michelina Festa Rosa Pasquinelli 1 Daniela Califano Giuseppe Palma Raffaele Costanzo 5 Barcaroli 6 Mario Capunzo 7 Renato Franco 8 Gaetano Rocco 9 Maria Pascale Caterina Turco 3,7 Vincenzo De Laurenzi 3,6 and Claudio Arra Functional Genomic Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori “Fondazione Giovanni Pascale”, IRCCS, Naples, Italy 2 Department of Pharmacy, University Salerno, Fisciano, 3 BIOUNIVERSA S.r.l., Animal Facility Medical Oncology Thoraco-Pulmonary Department, Experimental Clinical Sciences, G. D’Annunzio Fondazione D’Annunzio, Ce.S.I., Chieti, Medicine Surgery, Pathology Thoracic Surgery * These Authors contributed equally to the work Correspondence: Turco, email: Keywords : BAG3, SCLC, NSCLC, tumor growth, apoptosis Received July 08, 2014 Accepted 25, Published Abstract member HSP70 co-chaperones family, has been shown play a relevant role in survival, growth invasiveness different types. In this study, we investigate expression BAG3 66 specimens from lung tumors protein small cell cancer (SCLC) growth. Normal tissue did not express while detected by immunohistochemistry all 13 squamous carcinomas, adenocarcinomas large carcinomas. Furthermore, 22 36 SCLCs analyzed. The on SCLC survival was determined down-regulating levels two human lines, i.e. H69 H446, vitro measuring cisplatin induced apoptosis. Indeed down-regulation determines increased death sensitizes cells treatment. effect also investigated an vivo xenograft model treating mice with adenovirus expressing specific bag3 siRNA. Treatment siRNA-Ad significantly reduced improved animal survival. conclusion show that subset over exerts anti-apoptotic resulting resistance chemotherapy.