In Vitro Studies on Zinc Binding and Buffering by Intestinal Mucins.

摘要: The investigation of luminal factors influencing zinc availability and accessibility in the intestine is great interest when analyzing parameters regulating intestinal resorption. Of note, mucins were suggested to play a beneficial role zinc. Their exact binding properties, however, remain unknown impact these glycoproteins on human resorption has not been investigated detail. Thus, aim this study elucidate uptake into enterocytes its transfer blood. In present study, vitro properties analyzed using commercially available porcine secreted goblet cell line HT-29-MTX. molecular capacity average affinity demonstrates that contain multiple zinc-binding sites with biologically relevant within one mucin molecule. Zinc enterocyte Caco-2 was impaired by zinc-depleted mucins. Yet does represent their form lumen vivo under adequate conditions. fact, zinc-uptake studies presence differing degree saturation revealed buffering glycoproteins, indicating mucin-bound still for cells. Finally, three-dimensional cultures studied comparing Caco-2/HT-29-MTX co-culture conventional monoculture. Here, secreting co-cultures yielded higher fractional elevated transport rates, suggesting facilitate act as delivery system epithelium.