Proteomic Strategies to Characterize Signaling Pathways

摘要: Cells respond to external stimuli by transducing signals through a series of intracellular molecules and eliciting an appropriate response. The cascade events which the are transduced include post-translational modifications such as phosphorylation ubiquitylation in addition formation multi-protein complexes. Improvements biological mass spectrometry protein/peptide microarray technology have tremendously improved our ability probe proteins, protein complexes, signaling pathways high-throughput fashion. Today, single spectrometry-based investigation pathway has potential uncover large majority known intermediates painstakingly characterized over decades discovering number novel ones. Here, we discuss various proteomic strategies characterize provide protocols for phosphoproteomic analysis.