作者: P. H. Huang , A. Mukasa , R. Bonavia , R. A. Flynn , Z. E. Brewer
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摘要: … (pTyr100 (Cell Signaling Technology, Beverly, MA) in 200 μl of immunoprecipitation buffer (100 mM Tris/100 mM NaCl/1% Nonidet P-40, pH 7.4) for 8 h at 4C. Immobilized metal affinity …