Stem and Progenitor Cell Subsets Are Affected by JAK2 Signaling and Can Be Monitored by Flow Cytometry

摘要: Although extremely rare, hematopoietic stem cells (HSCs) are divisible into subsets that differ with respect to differentiation potential and cell surface marker expression. For example, we recently found CD86− CD150+ CD48− HSCs have limited for lymphocyte production. This could be an important new tool studying hematological abnormalities. Here, analyzed HSC a series of markers in JAK2V617F transgenic (Tg) mice, where the mutation is sufficient cause myeloproliferative neoplasia deficiency. Total numbers were elevated 3 20 fold bone marrow mice. Careful analysis suggested accumulation involved multiple subsets, but particularly those characterized as CD150HI CD18L°CD41+ excluding Hoechst dye. Real-Time PCR their revealed erythropoiesis associated gene transcripts Gata1, Klf1 Epor high. Flow cytometry analyses based on two schemes multipotent progenitors (MPP) also alteration by JAK2 signals. The low CD86 paralleled large reductions lymphoid progenitors, few produced functioned normally when sorted placed culture. Either conferred disease transplanted. Thus, flow can used observe influence abnormal signaling progenitor subsets. Markers similarly distinguish categories human might very valuable monitoring such conditions. They serve indicators fitness suitability transplantation.