Transmission and evolution of OXA-48-producing Klebsiella pneumoniae ST11 in a single hospital in Taiwan.

摘要: OBJECTIVES Epidemic spread of OXA-48-producing Klebsiella pneumoniae, mainly mediated by the transmission a blaOXA-48-carrying plasmid, has threatened global health during last decade. Since its introduction to Taiwan in 2013, OXA-48 become second most common carbapenemase. We described and evolution an OXA-producing K. pneumoniae clone single hospital. METHODS Twenty-two were isolated between October 2013 December 2015. Comparative genomic analysis was performed based on WGS data generated with Illumina MinION techniques. RESULTS Seventeen 22 that belonged ST11, same capsular genotype, KL64, differed from each other seven or fewer SNPs, considered outbreak strains. Eight 17 strains harboured 65499 bp IncL plasmid (called pOXA48). pOXA48 absent remaining nine Instead, 24.9 kb fragment integrated into prophage region their chromosomes. Transmission routes ST11_KL64 sublineages, which carried either chromosomally blaOXA-48, reconstructed. CONCLUSIONS Clonal expansion sublineages contributed nosocomial pneumoniae. The chromosome-borne blaOXA-48 lineage emerged 2 year period Dissemination OXA-48, is vertically transmitted even absence selective pressure antimicrobials, deserves public attention.