Standardisation of the quantitation of serum amyloid A protein (SAA) in human serum.
作者： Nicole L. Godenir , Mohammed S. Jeenah , Gerhard A. Coetzee , Deneys R. Van der Westhuyzen , Alistair F. Strachan
DOI: 10.1016/0022-1759(85)90243-1
关键词: Immunoradiometric assay 、 Coomassie blue 、 Chemistry 、 Serum Amyloid A Protein 、 Chromatography 、 High density 、 Standard curve
摘要: Abstract An adequate method for standardising the quantitation of serum amyloid A protein (SSA) in human was developed. Acute phase high density lipoprotien 3 (HDL ) used as a standard. The concentration SAA standard determined by use purified SAA. After determination, various concentrations were run on SDS-polyacylamide gel together with HDL containing an unknown amount amongst apolipoproteins. From curve obtained pyridine extraction (Coomassie blue colour yield at 605 nm) determined. established immunoradiometric assay (IRMA) standardised . normal and acute sera
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