Bio-fabrication of silver nanoparticles by Pseudomonas aeruginosa: optimisation and antibacterial activity against selected waterborne human pathogens.

摘要: Multiple drug resistance and treatment of contaminated water has become a serious issue in past years. Silver nanoparticles (AgNPs), being bactericidal, non-toxic, cheap environment friendly behaviour, have drawn attention to overcome these problems. This study been designed synthesise AgNPs from Pseudomonas aeruginosa. formation was confirmed by colour change UV-vis spectroscopy. Furthermore, Fourier transform infrared spectroscopy peaks demonstrated the presence capped proteins as reducing stabilising agent. Transmission electron microscopy micrograph revealed spherical shape with size ranging between 10 20 nm. Antibacterial activity evaluated against most prevalent waterborne pathogens enterohaemorrhagic Escherichia coli Salmonellae typhimurium. Moreover, antibacterial tested for which showed attenuation bacterial load within 8 h growth kinetics data. did not exhibit haemolytic effects on human red blood cells (RBCs) even at 100 mg L-1 concentration AgNPs. The results herein suggest that synthesised P. aeruginosa under optimised conditions microbicidal property having no toxic effect RBCs. These could be employed after process optimisation.