Genetic analysis of alpha-fetoprotein synthesis in mice.

摘要: The differential induction of alpha-fetoprotein (AFP) mRNA during liver regeneration in three inbred strains mice was examined to determine the genetic and molecular bases for differences protein production. BALB/cJ, C3H/He, C57BL/6 mice, previously identified as high, intermediate, low AFP producers, respectively, were used. Liver concentrations normal development after carbon tetrachloride administration measured shown correlate exactly with serum concentrations. By performing a series crosses, we two unlinked loci that acted independently affect inducibility mRNA. raf gene, by Olsson et al. (J. Exp. Med. 145:819-827, 1977), determines adult basal level mRNA, Rif gene affects its regeneration. using polymorphic restriction endonuclease site within albumin-AFP structural region, show neither regulatory is closely linked genes. In addition, concentration albumin or