A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes
作者： Gulnara Safina , Roland Ludwig , Lo Gorton
DOI: 10.1016/J.ELECTACTA.2009.10.052
关键词: Cellobiose dehydrogenase 、 Sample preparation 、 Lactose 、 Enzyme electrode 、 Analytical chemistry 、 Biosensor 、 Chemistry 、 Detection limit 、 Glutaraldehyde 、 Chromatography 、 Immobilized enzyme
摘要: A rapid and simple approach of lactose analysis is proposed based on 3rd generation amperometric biosensors employing cellobiose dehydrogenase (CDH) from Trametes villosa or Phanerochaete sordida immobilised screen-printed carbon electrodes (SPCEs). After optimisation the working conditions - pH carrier buffer, flow rate applied potential sensors were able to detect in a concentration range between 0.5-200 mu M 0.5-100 T. P. CDH, respectively. The limit detection 250 nM (90 g/L) for both. Biosensors SPCEs modified with multiwalled nanotubes showed higher sensitivity than unmodified SPCEs. Crosslinking glutaraldehyde poly(ethyleneglycol)diglycidyl ether improved not only stability but also analytical response. developed sensor has been successfully determination dairy (milk different percentages fat, lactose-free milk yogurt) good reproducibility (RSD = 1.5-2.2%). No sample preparation except dilution process needed. biosensor easy make operate, inexpensive reveals high reliability. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
elsevier.com
sci-hub.se 参考文章(29)
E. P. Medyantseva, N. Yu. Il'icheva, S. A. Eremin, G. K. Budnikov, Immunoextraction of Pesticide Residues from Different Classes Followed by Amperometric Determination Journal of Analytical Chemistry. ,vol. 58, pp. 639- 640 ,(2003) , 10.1023/A:1024786020786
Robert A. Meyers, Encyclopedia of analytical chemistry : applications, theory and instrumentation John Wiley. ,(2000)
Marek Trojanowicz, Ashok Mulchandani, Marco Mascini, Carbon Nanotubes‐Modified Screen‐Printed Electrodes for Chemical Sensors and Biosensors Analytical Letters. ,vol. 37, pp. 3185- 3204 ,(2004) , 10.1081/AL-200040320
K. Ravichandran, Richard P. Baldwin, Enhanced voltammetric response by electrochemical pretreatment of carbon paste electrodes Analytical Chemistry. ,vol. 56, pp. 1744- 1747 ,(1984) , 10.1021/AC00273A052
Eric Moore, Miloslav Pravda, George G. Guilbault, Development of a biosensor for the quantitative detection of 2,4,6-trichloroanisole using screen printed electrodes Analytica Chimica Acta. ,vol. 484, pp. 15- 24 ,(2003) , 10.1016/S0003-2670(03)00311-8
Naruhide Sato, Hirokazu Okuma, Development of single-wall carbon nanotubes modified screen-printed electrode using a ferrocene-modified cationic surfactant for amperometric glucose biosensor applications Sensors and Actuators B-chemical. ,vol. 129, pp. 188- 194 ,(2008) , 10.1016/J.SNB.2007.07.095
Michele Del Carlo, Marcello Mascini, Alessia Pepe, Gianfranco Diletti, Dario Compagnone, Screening of food samples for carbamate and organophosphate pesticides using an electrochemical bioassay Food Chemistry. ,vol. 84, pp. 651- 656 ,(2004) , 10.1016/J.FOODCHEM.2003.08.006
M.A. JAWAD, J. DORIE, N. EL MURR, Electrochemical Quantitative Analysis of Uric Acid in Milk Journal of Food Science. ,vol. 56, pp. 594- 595 ,(1991) , 10.1111/J.1365-2621.1991.TB05336.X
Tokuji Ikeda, Daisuke Kobayashi, Fumio Matsushita, Takamasa Sagara, Katsumi Niki, Bioelectrocatalysis at electrodes coated with alcohol dehydrogenase, a quinohemoprotein with heme c serving as a built-in mediator. Journal of Electroanalytical Chemistry. ,vol. 361, pp. 221- 228 ,(1993) , 10.1016/0022-0728(93)87058-4
Jarod Brain, Wendy Collier, Alan Hart, The occurrence and nature of electrochemical activity in milk from a herd of dairy cows. Journal of Dairy Research. ,vol. 73, pp. 115- 120 ,(2006) , 10.1017/S0022029905001676