Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against Clostridioides difficile

摘要: Clostridioides difficile is the main cause of health-care-associated infectious diarrhoea. Toxins, TcdA and TcdB, secreted by this bacterium damage colonic epithelial cells in severe cases culminates pseudomembranous colitis, toxic megacolon death. Vaccines human trials have focused exclusively on parenteral administration toxin-based formulations. These vaccines promote toxin-neutralising serum antibodies but fail to confer protection from infection gut. An effective route immunise against gut pathogens stimulate a protective mucosal antibody response (secretory immunoglobulin A, IgA) at site oral route. Additionally, immunisation generates systemic (IgG). Using route, two different antigens were tested hamster model: The colonisation factor CD0873 TcdB fragment. Animals immunised with generated significantly higher titre sIgA intestinal fluid IgG compared naive animals, which inhibited adherence C. Caco-2 cells. Following challenge hypervirulent isolate, CD0873-immunised group showed mean increase 80% time experimental endpoint animals. Survival body condition correlated bacterial clearance reduced pathology cecum. Our findings advocate as promising vaccine candidate difficile.