Electrostatic characterization of liposomes

摘要: Abstract A variety of experimental techniques can be used to probe the electrostatic characteristics lipid bilayers and liposomes. Many are based on titration charge-dependent liposome properties. Determination (headgroup) conformation, bilayer phase transition temperature, interbilayer force, mobility in external electric field, or transbilayer transport but a few examples this. Laser-Doppler velocimetry, dynamic light scattering by suspension, is probably best currently available tool for fast and/or routine determination potential liposomes; proviso this that data analysis done prudently. In addition potential-dependent adsorption binding ion ionic probes surface measured. Interesting organic ions made visible suitable (fluorescent, nuclear, paramagnetic, etc.) labels. The conclusions drawn from any ion-liposome interaction study only reliable, however, if Coulombic, as well non-trivial effects both accounted for. When magnitude latter not known theoretically, accuracy improved subtracting complementary set (measured with uncharged vesicles highly concentrated salt solution) corresponding results obtained charged liposomes at low concentration.