Vascular endothelial growth factor. A cytokine modulating endothelial function in rheumatoid arthritis.

摘要: Angiogenesis is important in the proliferation of inflammatory synovial tissue. Vascular endothelial growth factor (VEGF) an cell mitogen that also angiogenic vivo. We examined potential role VEGF mediating chemotaxis and cells rheumatoid arthritis (RA) using samples tissue fluid from 55 arthritic patients. Synovial by ELISA was higher RA fluids (386 +/- 122 ng/ml) (SE) compared with osteoarthritis (OA) (< 0.8 (p < 0.05) or patients other arthritides (6.6 2 ng/ml). In addition to its known mitogenic properties, we found human rVEGF chemotactic for HUVECs at concentrations above 0.02 nM. Incubation neutralizing anti-VEGF resulted 23 66% (mean 53 4%) inhibition HUVEC chemotaxis. Conditioned medium four five explants bovine adrenal capillary cells. Anti-VEGF neutralized 19 42% 28 this activity. To determine cellular source tissue, employed immunohistochemistry. VEGF+ were rarely 1%+) normal tissues. contrast, OA tissues exhibited lining (8% 13%, respectively). A few macrophages both (5% 2%, These results elucidate a newly described function as potent chemotaxin well RA-associated migration proliferation.