Cinnamyl Alcohol, the Bioactive Component of Chestnut Flower Absolute, Inhibits Adipocyte Differentiation in 3T3-L1 Cells by Downregulating Adipogenic Transcription Factors.

摘要: The extract of chestnut (Castanea crenata var. dulcis) flower (CCDF) has antioxidant and antimelanogenic properties, but its anti-obesity properties have not been previously examined. In this study, we tested the effect CCDF absolute on adipocyte differentiation by using 3T3-L1 cells determining bioactive component in cell differentiation. (0.1–100μg/mL) did change viability. At 50μg/mL 100μg/mL, significantly reduced accumulation lipid droplets that were induced culture medium containing 3-isobutyl-1-methylxanthine/dexamethasone/insulin (MDI). GC/MS analysis showed contains 10 compounds. Among these compounds, cinnamyl alcohol (3-phenyl-2-propene-1-ol) dose-dependently inhibited increased MDI-contained medium-cultured at a concentration range 0.1μg/mL to 10μg/mL cause cytotoxicity cells. inhibitory was significant 5μg/mL (70.27±1.50% response MDI alone-treated state, P<0.005) (28.94±1.76% P<0.005). Moreover, enhanced expression obesity-related proteins (PPARγ, C/EBPα, SREBP-1c, FAS) medium-cultivated attenuated addition 10μg/mL. These findings demonstrate suppresses inhibiting anti-adipogenesis-related proteins, it may be main absolute, exerting antidifferentiation action Therefore, alcohol, as well potential candidates for prevention or treatment obesity.