Molecular cloning and expression of GRK6. A new member of the G protein-coupled receptor kinase family.
作者： J.L. Benovic , J Gomez
DOI: 10.1016/S0021-9258(19)36546-9
关键词: Protein kinase A 、 Beta adrenergic receptor kinase 、 Kinase 、 Rhodopsin kinase 、 G-Protein-Coupled Receptor Kinase 5 、 G protein-coupled receptor kinase 、 Biology 、 Rhodopsin 、 Molecular biology 、 G protein
摘要: Abstract G protein-coupled receptor kinases (GRK), such as the beta-adrenergic kinase (beta ARK) and rhodopsin kinase, specifically phosphorylate activated form of receptors. To identify additional members GRK family, we screened a human heart cDNA library by low stringency hybridization using catalytic domains two beta ARK isoforms. Here report isolation that encodes 576-amino-acid protein termed GRK6, has significant homology with GRK5 (70.1% amino acid identity), IT11 (68.5%), (47.1%), (37.4%). RNA blot analysis GRK6 selected tissues reveals distinct mRNAs 3 2.4 kilobases distribution very similar to (i.e. brain, skeletal muscle > pancreas heart, lung, kidney, placenta liver). overexpressed in Sf9 insect cells baculovirus system, was able both 2-adrenergic stimulus-dependent fashion, although it significantly less active then on these substrates. These data extend family GRKs suggest may have substrate specificity quite from kinase.
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