Extractability of astaxanthin in a mixed culture of a carotenoid over-producing mutant of Xanthophyllomyces dendrorhous and Bacillus circulans in two-stage batch fermentation

摘要: The productivity and extractability of astaxanthin in a mixed culture Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) NCHU-FS501, an over-producing mutant, Bacillus circulans CCRC 11590 was evaluated 1.5 l fermentor using two-stage batch fermentation technique. first stage for X. cultivation. second the red yeast B. circulans. highest lytic enzyme activity found at 24 h with nitrogen base (YNB) as source incubation 30 °C pure culture. induction influenced by cell wall concentration dendrorhous. fastest observed addition 2.5 g/l wall. cultivation time significantly affected carotenoid fermentation. Minimum when cultivated 96 stage. Glucose 45 YNB supported high growth production, production 9010 during Although produced more peptone stage, optimal pH temperature pigment extraction were 6.5 30–34 °C, respectively. Under these conditions, about 97% total could be extracted after 48 time. When crude recycled culture, 20 units/ml obtained one cycle (48 h), 69% carotenoids extracted. © 2002 Elsevier Science Ltd. All rights reserved.