Electrochemical biosensor for the epithelial cancer biomarker EpCAM based on reduced graphene oxide modified with nanostructured titanium dioxide

摘要: An electrochemical immunosensor has been fabricated for the early determination of epithelial cell adhesion molecules (EpCAM, tumor biomarker) antigen using reduced graphene oxide (rGO) modified with nanostructured titanium dioxide (TiO2). The hydrothermally synthesized rGO@TiO2 nanocomposite electrophoretically deposited on indium tin (ITO) coated glass substrate, and deposition was confirmed various spectroscopic, microscopic, techniques. rGO@TiO2/ITO electrode shows improved electron transfer kinetics an rate constant 1.93 × 10−7 cm·s−1. Furthermore, electrodes were used covalent immobilization monoclonal EpCAM antibodies. Electrochemical the EpCAM cancer biomarker is achieved differential pulse voltammetry by scanning potential from − 0.4 to 0.8 V amplitude 50 mV. rGO@TiO2-based biosensor high sensitivity (3.24 μA·mL·ng−1·cm−2), wide detection range (0.01 ng·mL−1 60 ng·mL−1), low limit (0.0065 ng·mL−1, S/N = 3). highly stable regenerable successfully applied to the in spiked human serum samples.